Engineered virus-like particles for transient delivery of prime editor ribonucleoprotein complexes in vivo
- rani mizrahi
- Oct 29
- 1 min read
An article published in Nature Biotechnology presents an innovative approach to improving the applicability of Prime Editing – an advanced genome editing technology that enables the creation of precise changes such as substitutions, insertions, and deletions.
The main challenge so far has been in efficiently and safely transferring the editing components into living cells.
In this study, engineered virus particles (PE-eVLPs) were developed that allow for the transient delivery of Prime Editor ribonucleoprotein complexes along with the required RNA molecules.
Advanced versions of the particles (v3 and v3b) have achieved a significant improvement in editing efficiency – up to 170 times in human cells compared to previous models.
In mouse models of genetic blindness, a single injection of v3 PE-eVLPs resulted in therapeutic levels of editing, partial restoration of protein expression, and even improvement in visual function.
The findings demonstrate that this approach enables more efficient and safer delivery of Prime Editing components, while reducing risks of off-target editing and dangerous genetic integration – thus
